Propanc Biopharma, Inc. announced that successful production of a synthetic recombinant version of the proenzyme trypsinogen was completed via the Proenzyme Optimization Project 1 (POP1) joint research and drug discovery program. The program is designed to produce a backup clinical compound to the Company's lead product candidate, PRP, which is targeting metastatic cancer from solid tumors. The program's lead research scientist, Mr. Aitor González, was able to produce the recombinant trypsinogen successfully.

Previously, attempts to produce synthetic trypsinogen when manufacturing in larger quantities were unable to be stabilized. Mr. González consulted with Professor Diethard Mattanovich, Institute of Microbiology and Microbial Biotechnology, at the University of Natural Resources and Life Sciences, Vienna, Austria, Europe, and is ready to undertake a larger scale production of recombinant trypsinogen, subsequently purified to achieve a higher quality product. This process will also be repeated for recombinant chymotrypsinogen to manufacture a synthetic recombinant formulation to the Company's lead product candidate, PRP.

The objective of the POP1 program is to produce large quantities of trypsinogen and chymotrypsinogen for commercial use that exhibits minimal variation between lots and without sourcing the proenzymes from animals. Propanc is undertaking the challenging research project in collaboration with the Universities of Jaén and Granada, led by Mr. González, supported by Prof. Macarena Perán, Ph.D. and Prof. Juan Antonio Marchal M.D., representing the Universities, respectively, and Dr. Kenyon. PRP is a mixture of two proenzymes, trypsinogen and chymotrypsinogen from bovine pancreas administered by intravenous injection.

A synergistic ratio of 1:6 inhibits growth of most tumor cells. Examples include kidney, ovarian, breast, brain, prostate, colorectal, lung, liver, uterine and skin cancers.