They include two of the biggest U.S banks, J.P Morgan Chase and Citigroup, the world's largest hotel company, Marriot as well the Blue Cross Blue Shield Association, a federation of 36 independent companies that provide healthcare insurance.
It follows last Wednesday's violence in Washington, when President Trump supporters stormed the Capitol in an attempt to stop the formal recognition of Trump's defeat.
Five people died, including a police officer.
JPMorgan and Citigroup said they will pause all political action committee donations, over the coming months.
In a memo to employees seen by Reuters, Citigroup's global government affairs head, Candi Wolff said "We want you to be assured that we will not support candidates who do not respect the rule of law."
They had previously donated to Republican Senator Josh Hawley, who led the charge against the certification of Biden's win.
Other major U.S. companies like Ford and Walmart haven't paused donations yet, but a Walmart spokesman said they will factor in last week's events to their review process.
Meanwhile, the digital payments company Stripe said it would stop processing payments for Trump's campaign website following the riot.
And late Sunday the PGA tweeted that its board voted to strip the 2022 PGA golf championship away from the Trump National Bedminster golf club in New Jersey.
