BRUSSELS, May 22 (Reuters) - Apple has challenged at Europe's second-highest court a 1.84 billion euro ($2 billion) fine imposed by EU antitrust regulators for impeding Spotify and other streaming rivals via restrictions on its App Store, according to a court filing.

Apple had already said it would appeal after the European Commission handed down the fine in March, its first ever penalty for infringing EU antitrust rules and the third biggest penalty meted out to a company for anti-competitive behaviour.

A ruling by the Luxembourg-based General Court could take several years while an appeal against its judgment to the Court of Justice of the European Union, Europe's top court, could drag out the litigation by a few more years.

($1 = 0.9228 euros) (Reporting by Foo Yun Chee; Editing by Kirsten Donovan)